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KMID : 0370220160600010008
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Yakhak Hoeji
2016 Volume.60 No. 1 p.8 ~ p.14
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Effect of Corticosterone Pretreatment on the Production of LPS-Induced Inflammatory Mediators in Hepa1c1c-7 Cells
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채병숙:Chae Byeong-Suk
저자없음:No authors listed
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Abstract
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Endotoxemia induces production of inflammatory mediators and acute phase proteins, leading to multiorgan injury and systemic inflammation. Hypothalamic-pituitary-adrenal (HPA) axis activation and glucocorticoids (GCs) release modify endotoxemia-induced inflammatory responses. In the present study, we investigated whether pre-exposure of GCs influences endotoxin-induced production of inflammatory mediators in hepatocytes. Hepa1c1c-7 cells were pretreated with
low concentrations of corticosterone for 24 h and then cultured without corticosterone in the presence or absence of LPS.
Our results demonstrated that LPS alone significantly enhanced production of IL-6 and CRP but reduced vascular ndothelial growth factor (VEGF) compared to controls. Combination of corticosterone pretreatment and LPS significantly upregulated production of IL-6, IL-1β, and VEGF but downregulated CRP compared to those in LPS alone. These findings suggest that in low concentration of corticosterone-preexposed hepatocytes, endotoxemia may induce upregulation of IL-6, IL-1β, VEGF and but downregulation of CRP.
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KEYWORD
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corticosterone, IL-6, IL-1β, CRP, VEGF, endotoxin, hepatocyte
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